Stacking in lipid vesicle-tubulin mixtures is an artifact of negative staining.
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Stacking in lipid vesicle-tubulin mixtures is an artifact of negative staining
Multilamellar stacking seen in negatively stained lipid vesicle-tubulin mixtures has been attributed to lipid-protein interactions (Caron, J. M., and R. D. Berlin, 1979, J. Cell Biol. 81:665-671). We show that this stacking is produced by the phosphotungstic acid used for staining, independent of the presence of tubulin in the sample. The morphology of negatively stained single bilayer vesicles...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1980
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.86.3.881